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biotinylated anti mouse il 2rγ  (R&D Systems)


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    R&D Systems biotinylated anti mouse il 2rγ
    Biotinylated Anti Mouse Il 2rγ, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+mouse+il+2r%CE%B3/Mouse+c-Rel+Biotinylated+Antibody/pm37904191-100-4-8
    Average 91 stars, based on 6 article reviews
    biotinylated anti mouse il 2rγ - by Bioz Stars, 2026-09
    91/100 stars

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    Incubation:

    Article Title: NFAT1 and NFκB regulates expression of the common γ-chain cytokine receptor in activated T cells.
    Article Snippet: Cell lysates were obtained from stimulated LN T, EL4, and EL4 KO, EL4 mutant cell lines, resolved using sodium dodecyl sulphate–polyacrylamide gel electrophoresis (12% acrylamide; Invitrogen) under reducing conditions, and transferred to polyvinylidene difluoride membranes (Amersham Biosciences, Amersham, Buckinghamshire, U.K). .. Blots were incubated with biotinylated anti-mouse IL-2Rγ (1:200, R&D system), p65 (F-6), cRel (C), NFATc2 (4G6-G5) (1:1000, all from Santa Cruz Biotechnology), and GAPDH, phospho p65(ser536), phospho AKT (ser473), phospho MEK1/2 (Ser217/221) (1:1000, Cell Signaling, Danvers, Massachusetts, USA) antibodies, followed by horseradish peroxidase (HRP)-conjugated streptavidin (1:2000, BioLegend) or HRP-conjugated anti-rabbit or anti-mouse IgG (1:5000, Cell Signaling) and HRP-conjugated anti-mouse β-actin antibodies (1:2000, Santa Cruz Biotechnology). .. The membranes were then incubated with enhanced chemiluminescence to determine the reagents (AmershamTM, Amersham, Buckinghamshire, U.K) and exposed using the LAS-3000 Imaging system (Fujifilm, Minato-ku, Tokyo, Japan) and Imager 680 (AmershamTM).



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